Journal: Molecular Cancer

Article Title: Variation in transcriptional regulation of cyclin dependent kinase inhibitor p21 waf1/cip1 among human bronchogenic carcinomas

doi: 10.1186/1476-4598-4-23

Figure Lengend Snippet: Transcript abundance measurements for cultured normal BEC and BC cell lines

Article Snippet: The reagents for analysis of E2F1 and p21 were commercially prepared (Gene Express, Inc., Toledo, OH).

Techniques: Cell Culture

Journal: Molecular Cancer

Article Title: Variation in transcriptional regulation of cyclin dependent kinase inhibitor p21 waf1/cip1 among human bronchogenic carcinomas

doi: 10.1186/1476-4598-4-23

Figure Lengend Snippet: Descriptive statistics for E2F1, p73α, and p21 transcript abundance measurements

Article Snippet: The reagents for analysis of E2F1 and p21 were commercially prepared (Gene Express, Inc., Toledo, OH).

Techniques:

Journal: Molecular Cancer

Article Title: Variation in transcriptional regulation of cyclin dependent kinase inhibitor p21 waf1/cip1 among human bronchogenic carcinomas

doi: 10.1186/1476-4598-4-23

Figure Lengend Snippet: Lack of correlation between E2F1 and p21 TA . There was no correlation between E2F1 and p21 TA among BC cell lines (N = 14) and primary BC tissues (N = 7). Each point represents the mean value from triplicate measurements of E2F1 and p21 as shown in Tables 1 and 2 (except where indicated).

Article Snippet: The reagents for analysis of E2F1 and p21 were commercially prepared (Gene Express, Inc., Toledo, OH).

Techniques:

Journal: Molecular Cancer

Article Title: Variation in transcriptional regulation of cyclin dependent kinase inhibitor p21 waf1/cip1 among human bronchogenic carcinomas

doi: 10.1186/1476-4598-4-23

Figure Lengend Snippet: Bivariate analysis of p73α and p21 TA . p73α and p21 TA were positively correlated (p < 0.05) among BC cell lines (N = 7) where p53 was known to be inactivated by mutation or deletion and p73 was wild type. Each point represents the mean value from triplicate measurements of p73α and p21 as shown in Table 1.

Article Snippet: The reagents for analysis of E2F1 and p21 were commercially prepared (Gene Express, Inc., Toledo, OH).

Techniques: Mutagenesis

Journal: Molecular Cancer

Article Title: Variation in transcriptional regulation of cyclin dependent kinase inhibitor p21 waf1/cip1 among human bronchogenic carcinomas

doi: 10.1186/1476-4598-4-23

Figure Lengend Snippet: Effect of p73α transient expression on p73α, p21, and E2F1 TA in Calu-1 cells . A) p73α TA was induced by over 3 orders of magnitude relative to mock transfected cells. Calu-1 cells were transfected with 5 μg of GFP control plasmid or HA-p73α. B) Total HA-p73 protein was analyzed in mock or p73α transfected cells. 20 μg of lysate were blotted on a PDVF membrane and incubated with an anti-hemagglutinin primary antibody conjugated to HRP. C) p21 was upregulated by 90% and E2F1 was reduced by 20% in p73α transfected cells. Results represent the mean value from triplicate measurements from three independent experiments. Error bars represent the S.E.M. RNA was extracted 24 hours post-transfection and treated with DNaseI. RNA was PCR amplified to rule out the possibility of plasmid contamination. No PCR products were detected.

Article Snippet: The reagents for analysis of E2F1 and p21 were commercially prepared (Gene Express, Inc., Toledo, OH).

Techniques: Expressing, Transfection, Control, Plasmid Preparation, Membrane, Incubation, Amplification